Department of General Zoology and Neurobiology (W3)
Institute: Institute for Biology and Biotechnology
Faculty of Biology and Biotechnology
44780 Bochum, Germany
Phone: +49 234 32 24363
Mail: Stefan.Herlitze@rub.de
Website: www.ruhr-uni-bochum.de/neurobiol/

 

Aim:  Visualization and temporal resolution of 5-HT receptor subtype participation in subcellular receptor domains of cortical neuron processing

GPCRs activated by monoaminergic neurotransmitters are often co-expressed within one neuron and on the pre- and postsynaptic sites. This necessitates spatial and temporal integration of GPCR signals within one neuron to elicit a specific modulatory effect. For example, 5-HT1A (Gi/o) and 5-HT2A (Gq/11) receptors are co-expressed in cortical pyramidal neurons and parvalbumin-positive interneurons (Jancke et al., 2021). 5-HT1A receptors are more closely located to the soma and have a higher affinity for 5-HT in comparison to 5-HT2A receptors, which are located to the proximal and distal dendrites. 5-HT1A and 5-HT2A receptors induce antagonistic modulation of neuronal activity. This raises the question as to how these different signals spatially and timely integrate in specific subcellular receptor domains, such that neuronal activity is specifically decreased or increased?

 

 10 selected publications 

• Karapinar R, Schwitalla JC, Eickelbeck D, Pakusch J, Mücher B, Grömmke M, Surdin T, Knšpfel T, Mark MD, Siveke I, Herlitze S. (2021) Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks. Nature Commun. 12(1):4488. doi: 10.1038/s41467-021-24718-0. PMID: 3430194

• Eickelbeck, D., Karapinar, R., Jack, A., Suess, S.T., Barzan, R., Azimi, Z., Surdin, T., Grömmke, M., Mark, M.D., Gerwert, K., Jancke, D., Wahle, P., Spoida, K. and Herlitze, S. (2019). CaMello-XR enables visualization and optogenetic control of Gq/11 signals and receptor trafficking in GPCR-specific domains. Commun. Biol. 2:60. doi: 10.1038/s42003-019-0292-y. eCollection 2019

• Spoida, K., Eickelbeck, D., Karapinar, R., Eckhardt, T., Mark, M.D., Jancke, D., Ehinger, B., Kšnig, P., Dalkara, D., Herlitze, S. and Masseck, O.A. (2016) Melanopsin Variants as Intrinsic Optogenetic On and Off Switches for Transient versus Sustained Activation of G Protein Pathways. Current Biology, 26(9):1206-12.

• Spoida, K., Masseck, O.A., Deneris, E.S. and Herlitze, S. (2014). Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice. Proc. Natl. Acad. Sci., USA, 111(17):6479-84.

• Masseck, O.A., Spoida, K., Dalkara, D., Maejima, T., Rubelowski, J.M., Wallhorn, L., Deneris, E.S. and Herlitze, S. (2014) Vertebrate cone opsins enable sustained and highly sensitive rapid control of Gi/o signaling in anxiety circuitry. Neuron 19(81): 1263-73.

• Mark, M.D., Maejima, T., Kuckelsberg, D., Yoo, J.W., Hyde, R., Shah, V., Gutierrez, D., Moreno, R.L., Kruse, W., Noebels, J. and and Herlitze, S. (2011). Delayed postnatal loss of P/Q type calcium channels recapitulates the absence epilepsy, dyskinesia, and ataxia phenotypes of genomic Cacna1A mutations. J. Neuroscience, 31(11):4311-26.

• Armbruster, B.N., Li, X., Pausch, M.H., Herlitze, S. and Roth, B.L. (2007). Evolving the lock to fit the key: using directed molecular evolution to create a family of muscarinic acetylcholine receptors to potently activated by an inert ligand. Proc. Natl. Acad. Sci., USA, 04(12):5163-8.

• Han, J., Mark, M.D., Waka, S., Rettig, J., Herlitze, S. (2006). RGS2 determines short-term synaptic plasticity in hippocampal neurons via regulation of Gi/o mediated presynaptic Ca2+ channel inhibition. Neuron, 51, 575-586.

• Li, X., Gutierrez, D., Hanson, G., Han, J., Mark, M.D., Chiel, H., Hegemann, P., Landmesser, L.T., Herlitze, S. (2005). Fast non-invasive control of neuronal excitability and network behavior by vertebrate rhodopsin and green algae channelrhodopsin. Proc. Natl. Acad. Sci., USA, 102, 17816-17821.

• Herlitze, S., Garcia, D.E., Mackie, K., Hille, B., Scheuer, T. and Catterall, W.A. (1996). Modulation of Ca2+ channels by G-protein bg subunits. Nature 380, 258-262.